EasySep™ EasySep™ 。 7-AAD,。, EasySep™ 。 ±,n = 3 – 7。 3. …
. EasySep™ Magnet（ST-18000）は、EasySepをいたのためのです。. のにじるは、EasySep™ Magnetic Particlesでラベルされたをカラムをいずするためになさをしています。. は、5 mL (12 x 75 mm)の ...
Using the EasySep was not very effective. On the contrary, using Magnisort MACS beads and respective a-CD14 from Affymetrix-eBioscience resulted in …
EasySep™T, T。. (CD11b, CD19, CD24, CD45R/B220, CD49b, TER119)（RapidSpheres™）。. EasySep™, …
The EasySep™ human T cell isolation kit is designed to isolate T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection.Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function.
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EasySepをいたウィルスにするの したからウイルスまたはウイルスをすることは、ウイルスのとのメカニズムをらかにするうえでなです。 サンプルをのですることで、よりいのをることができます。 EasySep™をしますとに、かつ、わずか8ので …
EASYSEP™ MAGNETS STEP INSTRUCTIONS EasyEights™ (Catalog #18103) Easy 50 5 mL tube 14 mL tube (Catalog #18002) 1 Prepare sample within the volume range. 1 - 2 mL1 - 6 5 - 25 Add sample to required tube. polystyrene round-bottom …
stem cellMACS：. 、EasySep®,；. 、StemSep®,；. 、RosetteSep®,,；. 、SpinSep® 。. 。. 、EasySep® ...
Typical EasySep™ Mouse Naïve CD4+ T Cell Isolation Profile. Starting with mouse splenocytes from an uninfected mouse, the naïve CD4+ T cell (CD4+CD44lowCD62Lhigh) content of the isolated fraction typically ranges from 89.9 - 95.6%. In the example above, the final purities of the start and isolated fractions are 14.3% and 94.7%, respectively.
EasySep™ Mouse CD138 Positive Selection Kit Table 2. EasySep™ Mouse CD138 Positive Selection Kit Protocol EASYSEP™ MAGNETS STEP INSTRUCTIONS EasyEights™ (Catalog #18103) 5 mL tube 14 mL tube 1 Prepare sample at the indicated cell concentration within the volume range and keep cold until use. 1 x 10^8 cells/mL 0.5 - 2 mL 1 x 10^8 cells/mL
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Then, I use the EasySep Kit to isolate the CD19+ cells. The purity is supposed to be ~99%, but I get much less (only once managed to …
EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system—for fast and easy isolation of highly purified cells that are ready for downstream applications. This versatile immunomagnetic cell separation technology enables positive selection, negative selection, or cell depletion.
Description. The EasySep™ Mouse Neutrophil Enrichment Kit is designed to isolate neutrophils from single-cell suspensions of bone marrow or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-neutrophils. Labeled cells are then recognized by Tetrameric ...
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EasySep™ Mouse Neutrophil Enrichment Kit（ST-19762）は、あるいはののシングルセルからをネガティブセレクションでするためにデザインされています。. なは、にするビオチンでターゲットされます。. ...
Figure 1. Typical EasySep™ Human Monocyte Isolation Profile. Starting with PBMCs prepared from human whole peripheral blood, the monocyte cell content (CD14+CD45+) of the isolated fraction obtained without (middle plots) or with EasySep™ Human Platelet Removal Cocktail (bottom plots) is typically 89.7 ± 3.4% and 87.3 ± 4.5%, respectively (gated on CD45, mean ± …
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Directions for Use – Manual EasySep™ Protocols See page 1 for Sample Preparation and Recommended Medium. Refer to Tables 1 and 2 for detailed instructions regarding the EasySep™ procedure for each magnet. Table 1. EasySep™ Human B Cell Enrichment Kit Protocol EASYSEP™ MAGNETS STEP INSTRUCTIONS EasySep™ (Catalog #18000) "The …
されたは、カラムフリーのEasySep™ magnetによりされます。 のは、しいチューブにぎれることでします。 は、EasySep™ Mouse CD4+ T Cell Enrichment Kit (ST-19752)のよりなとしておいいただけます。
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The volume of blood that can be processed depends on the EasySep™ magnet used for the isolation procedure. Blood samples must be placed in the required tube to properly fit into the appropriate EasySep™ magnet. To avoid loss of monocytes, EDTA must be added to the whole blood sample to a final concentration of 6 mM prior to labeling and ...
This product can be used in place of the EasySep™ Human T Cell Enrichment Kit (Catalog #19051) for even faster cell isolations. Advantages • Fast, easy-to-use and column-free • Up to 98% purity with high recovery • Untouched, viable cells Components EasySep™ Human T Cell Isolation Kit (Catalog #17951) EasySep™ Human T Cell Isolation Cocktail, 1 mL
Directions for Use – Manual EasySep™ Protocols See page 1 for Sample Preparation and Recommended Medium. Refer to Tables 1, 2, and 3 for detailed instructions regarding the EasySep™ procedure for each magnet. Table 1. EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Protocol EASYSEP™ MAGNETS STEP INSTRUCTIONS EasySep™
Typical EasySep™ Mouse Naïve CD4+ T Cell Isolation Profile. Starting with mouse splenocytes from an uninfected mouse, the naïve CD4+ T cell (CD4+CD44lowCD62Lhigh) content of the isolated fraction typically ranges from 89.9 - 95.6%. In the example above, the final purities of the start and isolated fractions are 14.3% and 94.7%, respectively.
Our unique cell separation portfolio combines our proven magnetic cell isolation technology with exciting new options, providing for workflows across basic and clinical research. Whether isolating cells in small-scale experiments or in high-throughput industrial settings – we offer manual, semi-automated, automated, and robotic integration ...